Journal: Frontiers in Neuroscience

Article Title: The 5-HT 6 Receptors in the Ventrolateral Orbital Cortex Attenuate Allodynia in a Rodent Model of Neuropathic Pain

doi: 10.3389/fnins.2020.00884

Figure Lengend Snippet: Microinjection of the selective 5-HT 6 receptor agonists into the contralateral VLO reduced SNI-induced allodynia. (A) Scatter plots showing no difference of PWT between naïve and sham animals. (B,E) Time-course curves showing the inhibitory effect of different doses of EMD-386088 and WAY-208466 on allodynia induced by SNI. (C,F) Scatter plots showing the inhibitory effect of different doses of EMD-386088 and WAY-208466 on allodynia during the 60-min observation period. (D) The locomotor activity in an open-field test was not affected after microinjection of 10 μg EMD-386088 into the VLO. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with the vehicle group; # p < 0.05, ## p < 0.01, and ### p < 0.001, compared with the 2.5 μg EMD group. n = 6–15 rats/group.

Article Snippet: After washing in PBS, sections were incubated with primary antibody anti-rabbit 5-HT 6 receptor (Bioss, bs-12058R, 1:1,000, Beijing, China) and anti-goat neuronal glutamate transporter (EMD Millipore, AB1520, 1:8,000, Temecula, CA, United States) for 48 h at 4°C.

Techniques: Activity Assay

Journal: Frontiers in Neuroscience

Article Title: The 5-HT 6 Receptors in the Ventrolateral Orbital Cortex Attenuate Allodynia in a Rodent Model of Neuropathic Pain

doi: 10.3389/fnins.2020.00884

Figure Lengend Snippet: The expression of 5-HT 6 receptors protein level was specifically decreased in the contralateral VLO after SNI. (A) Representative Western blotting and (B) summary scatter plots showing the 5-HT 6 receptors of the VLO in control (sham) and SNI rats. ** p < 0.01 and ## p < 0.01 vs corresponding control and contralateral group, respectively. n = 4 rats/group. 5-HT6R, 5-HT 6 receptors.

Article Snippet: After washing in PBS, sections were incubated with primary antibody anti-rabbit 5-HT 6 receptor (Bioss, bs-12058R, 1:1,000, Beijing, China) and anti-goat neuronal glutamate transporter (EMD Millipore, AB1520, 1:8,000, Temecula, CA, United States) for 48 h at 4°C.

Techniques: Expressing, Western Blot

Journal: Frontiers in Neuroscience

Article Title: The 5-HT 6 Receptors in the Ventrolateral Orbital Cortex Attenuate Allodynia in a Rodent Model of Neuropathic Pain

doi: 10.3389/fnins.2020.00884

Figure Lengend Snippet: Effects of the selective 5-HT 6 receptor antagonist SB-258585 on EMD- and WAY-induced anti-allodynia. (A,C) Time course curves showing the antagonizing effect of SB-258585 on EMD- and WAY-induced inhibition of allodynia. (B,D) Scatter plots showing the antagonizing effect of SB-258585 on EMD- and WAY-induced inhibition of allodynia during the 60-min observation period. (E) Time-course curves showing no effect of different doses of SB-258585 on allodynia induced by SNI. (F) Scatter plots showing no effect of different doses of SB-258585 on allodynia during the 60-min observation period. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with the vehicle group; # p < 0.05, ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared with the 10 μg EMD and 8 μg WAY group. n = 6–12 rats/group.

Article Snippet: After washing in PBS, sections were incubated with primary antibody anti-rabbit 5-HT 6 receptor (Bioss, bs-12058R, 1:1,000, Beijing, China) and anti-goat neuronal glutamate transporter (EMD Millipore, AB1520, 1:8,000, Temecula, CA, United States) for 48 h at 4°C.

Techniques: Inhibition

Journal: Frontiers in Neuroscience

Article Title: The 5-HT 6 Receptors in the Ventrolateral Orbital Cortex Attenuate Allodynia in a Rodent Model of Neuropathic Pain

doi: 10.3389/fnins.2020.00884

Figure Lengend Snippet: The blocking effects of the non-selective glutamate receptor antagonist KA on EMD-induced anti-allodynia in the VLO. (A) The colocalization of 5-HT 6 receptors and EAAC1 in the VLO. Scale bar, 50 μm; n = 4 rats/group. (B) Time course curves showing the antagonizing effect of KA on EMD-induced inhibition of allodynia. (C) Scatter plots showing the antagonizing effect of KA on EMD-induced inhibition of allodynia during the 60-min observation period. *** p < 0.001 and **** p < 0.0001, compared with vehicle group; # p < 0.05, ### p < 0.001, and #### p < 0.0001 compared with 10 μg EMD. n = 8–11 rats/group.

Article Snippet: After washing in PBS, sections were incubated with primary antibody anti-rabbit 5-HT 6 receptor (Bioss, bs-12058R, 1:1,000, Beijing, China) and anti-goat neuronal glutamate transporter (EMD Millipore, AB1520, 1:8,000, Temecula, CA, United States) for 48 h at 4°C.

Techniques: Blocking Assay, Inhibition

Journal: Frontiers in Neuroscience

Article Title: The 5-HT 6 Receptors in the Ventrolateral Orbital Cortex Attenuate Allodynia in a Rodent Model of Neuropathic Pain

doi: 10.3389/fnins.2020.00884

Figure Lengend Snippet: A schematic representation of the mechanisms underlying 5-HT 6 receptors’ regulation of SNI-induced mechanical allodynia. SNI-induced allodynia downregulates 5-HT 6 receptors specifically in the contralateral VLO. The downregulation of 5-HT 6 receptors, some of which are postsynaptically present in the glutamatergic neurons in the VLO, might subsequently decrease glutamate release via an AC/PKA cascade, thus resulting in allodynia. Therefore, selective 5-HT 6 receptor agonists may ameliorate some of these mal-adaptations by activation of 5-HT 6 receptors. GluR, glutamate receptors; 5-HT6R, 5-HT 6 receptors.

Article Snippet: After washing in PBS, sections were incubated with primary antibody anti-rabbit 5-HT 6 receptor (Bioss, bs-12058R, 1:1,000, Beijing, China) and anti-goat neuronal glutamate transporter (EMD Millipore, AB1520, 1:8,000, Temecula, CA, United States) for 48 h at 4°C.

Techniques: Activation Assay